In-situ observations by electron microscope or optical method

Electron Microscope Observations on Intracellular

Relatively few malignant neoplasms have been described in amphibia. The one that has been studied most extensively is the Luck6 renal adenocarcinoma of the frog, Rana pipiens. This malignant tumor occurs in from two to four per cent of frogs of this species, collected in the Lake Champlain region of northern Vermont and adjoining areas of Quebec. In a series of papers Luck6 described its histop...

Electron Microscope Observations on Frozen-dried Cells

To explore the problem of artefacts which may be produced during usual fixation, dehydration, and embedding, the authors have examined pancreas, liver, and bone marrow frozen at about -180 degrees C., dried, at -55 to -60 degrees C., embedded in methacrylate, sectioned, and floated on a formol-alcohol mixture. By these treatments the labile structure of living cells can be fixed promptly and em...

Electron microscope observations on mitotic chromosomes.

A method is described by which single mitotic cells growing in tissue culture can be selected under the light microscope and then sectioned for electron-microscopic study. The method has been applied to mitotic cells in newt heart cultures and to monolayer cultures of human fibroblasts. The structure of the chromosomes and of the mitotic apparatus at various phases of mitosis are described and ...

In Situ Experiments in the Scanning Electron Microscope Chamber

Since the first scanning electron microscope by Knoll (1935) and theoretical developments by von Ardenne (1938a, b) in the 30’s, this imaging technique has been widely used by generations of searchers from all the scientific domains to characterize the inner structure of matter. Even if the obtained information is essential for matter description or comprehension of matter transformation, the m...

In situ laser processing in a scanning electron microscope